Adenylate energy charge in Escherichia coli CR341T28 and properties of heat-sensitive adenylate kinase

Stabilization of adenylate energy charge by the adenylate deaminase reaction.

Functional capacities and the adenylate energy charge in Escherichia coli under conditions of nutritional stress.

Functional capacities in Escherichia coli cells starved for glucose were examined by comparing protein synthesis, utilization of new substrates, and maintenance of viability with the adenylate energy charge of the culture. When growth ceased because of glucose exhaustion in an E. coli culture, the energy charge dropped from 0.90 to about 0.80. During this time, the viable-cell count and the cap...

Circular dichroism investigation of Escherichia coli adenylate kinase.

We examined by circular dichroism (CD) spectroscopy in far- and near-ultraviolet three different molecular forms of Escherichia coli adenylate kinase: the wild type protein, the enzyme carboxymethylated at a single cysteine residue (Cys-77), and the thermosensitive adenylate kinase. The thermosensitive enzyme differs from the wild type protein in that a serine is substituted for a proline resid...

Adenylate energy pool and energy charge in maturing rape seeds.

A study of energy state and chemical composition of pod walls and seeds of maturing rape (Brassica napus L.) was conducted on two varieties, Victor and Gorczanski. Total adenosine phosphates, ATP, and adenylate energy charge increased with increasing cell number and cellular synthesis during the early stages, remained high at maximum dry weight accumulation and maximum substrate influx time, an...

Biochemical properties of hormone-sensitive adenylate cyclase.

PERSPECTIVES AND SUMMARY .......................................................................... 534 PROTEIN COMPONENTS OF HORMONE-SENSITIVE ADENYLATE CYCLASE ............................................................................................ 535 Hormone Receptors and .4denylate Cy¢lase ............................................................ 535 Hormone Receptors Linked to Aden...